normal human epidermal keratinocytes nhek  (PromoCell)

Journal: Communications Biology

Article Title: The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth

doi: 10.1038/s42003-026-10272-2

Figure Lengend Snippet: a MYPOP expression in normal (N) and tumor (T) cells detected by Western blotting showing endogenous protein levels of MYPOP in normal human epidermal keratinocytes (NHEK) and cervical cancer cells (HeLa). In addition, the expression of GFP and MYPOP in HeLa cells after transfection with pcDNA3.1-MYPOP (MYPOP), pEGFP-C3-MYPOP (GFP-MYPOP) or corresponding controls (pcDNA3.1, Control and pEGFP-C3, GFP-Control) is shown. MYPOP and GFP were stained using anti-MYPOP pAb and anti-GFP mAb. GAPDH staining was used as a loading control. b – i GFP-Control or GFP-MYPOP expressing HeLa cells. b , c Lower ( b ) and higher ( c ) magnification for representative fluorescence microscopy images of GFP-Control or GFP-MYPOP (green) expressing cells 24 h post transfection (p.t.). Cell nuclei were stained using Hoechst 33342 (blue). d , e Representative fluorescence microscopy images treated as in ( c ) showing cytoplasmic, nucleocytoplasmic ( d ) and nuclear ( e ) localization as well as co-localization of GFP-MYPOP (green) and DNA (blue). f Co-localization analysis between GFP or GFP-MYPOP and DNA (Hoechst) using Pearson correlation coefficient (PCC). At least 10 GFP-positive cells were analyzed for each treatment and biological replicate ( n = 3). Values are shown as mean + SD. Statistical significance was determined with p = 0.0001 comparing GFP-MYPOP and GFP-Control. g Representative fluorescence microscopy images of GFP-MYPOP (green) expressing cells showing shrunken or fragmented nuclei at 24 h p.t. Cell nuclei (blue) as above. h , i Quantification of fragmented and shrunken nuclei at 24 h p.t ( h ) and 48 h p.t ( i ). At least 100 GFP-positive cells were analyzed for each treatment, time point and biological replicate, respectively. Values ( n = 3) are shown as mean + SD. Statistical significance was determined with p (24 h p.t.) = 0.0036 and p (48 h p.t.) = 0.0428 comparing GFP-MYPOP and GFP-Control.

Article Snippet: Normal Human Epidermal Keratinocytes (NHEK) were purchased from PromoCell, Germany and were cultivated according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Transfection, Control, Staining, Fluorescence, Microscopy

Journal: Communications Biology

Article Title: The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth

doi: 10.1038/s42003-026-10272-2

Figure Lengend Snippet: a Endogenous protein levels of MYPOP in untreated cancer cell lines. Normal skin cells (NHEK) and normal lung cells (181576 N, 181652 N) served as controls. GAPDH or β-actin staining was used as loading control. b Cancer cells were transfected with either a MYPOP expression plasmid or a control plasmid, selected for 6–12 days with G418, fixed, and stained with crystal violet. The cell-covered area was quantified (relative area). Values are presented as mean + SD. The mean for control-transfected cells was set to 100% (dotted line). Statistical significance was determined by comparing control and MYPOP expressing cells with p = 0.0022 for Huh7 ( n = 3), p = 0.0028 for HEK293 ( n = 5), p = 0.0064 for MCF7 ( n = 4), p = 0.0294 for HCT116 ( n = 3), p = 0.0106 for HeLa ( n = 3), p = 0.0003 for A549 ( n = 3), and p = 0.0026 for 2106 T ( n = 4).

Article Snippet: Normal Human Epidermal Keratinocytes (NHEK) were purchased from PromoCell, Germany and were cultivated according to the manufacturer’s instructions.

Techniques: Staining, Control, Transfection, Expressing, Plasmid Preparation

Journal: Communications Biology

Article Title: The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth

doi: 10.1038/s42003-026-10272-2

Figure Lengend Snippet: a Protein expression of untreated, control mRNA and MYPOP mRNA-transfected HeLa cells and NHEK cells, as indicated at different time points post transfection (6 h–54 h) was analyzed by western blotting using anti-MYPOP and anti-GAPDH antibodies. Under conditions of low protein loading and short exposure times, endogenous MYPOP is not detected in NHEK, as these parameters are optimized to prevent oversaturation of overexpressed MYPOP at 6 h p.t. Detection of endogenous MYPOP increases at later time points, reflecting cell growth and higher total protein content. Lower panel: optical microscope overview images of untreated, control mRNA and MYPOP mRNA-transfected HeLa cells (left panel) and NHEK cells (right panel) at 6 h and 54 h after mRNA transfection. b Growth curves (object counts per image, measurement every 2 h) of untreated, control mRNA and MYPOP mRNA-transfected HeLa, NHEK, CaSki, HaCaT, HCT116, and 2106 T cells at the indicated time points. Statistical significance ( n = 4 for HeLa, n = 3 for all others) was determined between Control and MYPOP cell counts at 70 h or 72 h p.t. as indicated with p = 0.0203 for HeLa, p = 0.0441 for CaSki, p = 0.4720 for HCT116, p = 0.2424 for NHEK, p < 0.0001 for HaCaT, and p = 0.0152 for 2106 T. c Left: MYPOP and GAPDH protein expression in Hela wild-type (WT) and MYPOP knockout (KO) cells. Statistical significance ( n = 3) between WT and KO cells was determined with p = 0.0014 for MYPOP band intensities. Center: optical microscope overview images of HeLa WT and KO cells. Right: growth curve of HeLa WT and KO cells. Statistical significance ( n = 4) was determined with p = 0.6099 at 72 h t.p.

Article Snippet: Normal Human Epidermal Keratinocytes (NHEK) were purchased from PromoCell, Germany and were cultivated according to the manufacturer’s instructions.

Techniques: Expressing, Control, Transfection, Western Blot, Microscopy, Knock-Out

Journal: Communications Biology

Article Title: The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth

doi: 10.1038/s42003-026-10272-2

Figure Lengend Snippet: Volcano plots depicting gene expression changes of control and MYPOP mRNA-transfected HeLa and NHEK cells at 6 h and 24 h p.t. as indicated. The labels display the overlapping DEGs, which were selected from the initial RNA-Seq experiment shown in Fig. , comprising the 30 top DEGs (15 up, 15 downregulated) and the 27 ‘cell cycle’ genes. Significantly downregulated candidates with adjusted p ≤ 0.05 are shown in blue, and upregulated candidates with adjusted p ≤ 0.05 are shown in red.

Article Snippet: Normal Human Epidermal Keratinocytes (NHEK) were purchased from PromoCell, Germany and were cultivated according to the manufacturer’s instructions.

Techniques: Gene Expression, Control, Transfection, RNA Sequencing

Journal: Applied and Environmental Microbiology

Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

doi: 10.1128/aem.01549-25

Figure Lengend Snippet: Dose-dependent effects on cytokine secretion. NHEKs (p3–5) cultured to 80% confluency were irradiated at 17–33 mJ/cm². Supernatants were collected 24 h post-irradiation and assessed via cytokine array ( A ) or via ELISA for IL-6 ( B ), IL-8 ( C ), CCL20 ( D ), and TNF-α ( E ). ELISA data are presented as mean ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

Techniques: Cell Culture, Irradiation, Enzyme-linked Immunosorbent Assay

Journal: Applied and Environmental Microbiology

Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

doi: 10.1128/aem.01549-25

Figure Lengend Snippet: Exposure to a skin PMC elevates cytokine secretion regardless of UVB exposure. NHEKs (p2–5) were treated with PBS (PBS, no UVB) or S. epidermidis, S. hominis, M. luteus, C. tuberculostearicum, and C. acnes for 30–90 min and subsequently exposed to UVB (33 mJ/cm²). Supernatants were collected 24 h post-inoculation with each organism, and the effects of the skin microbial PMC ± UVB on IL-6 ( A ), IL-8 ( B ), TNF-α ( C ), and CCL20 ( D ) secretion were assessed using ELISA. ** P < 0.01 and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Applied and Environmental Microbiology

Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

doi: 10.1128/aem.01549-25

Figure Lengend Snippet: UVB impacts the relative abundance of skin commensals in a polymicrobial skin community. NHEKs (p2–5) were incubated with a polymicrobial community for 90 min, media replaced, and cultures exposed to 33 mJ/cm2 UVB or left unirradiated (no UVB). Polymicrobial community composition was assessed 24 h post-treatment via qPCR, where relative proportions of each organism were calculated via species-specific standard curves and presented as collated data ( A , results for C. tuberculostearicum and M. luteus are not visible in the graph, as abundance was <1% of the total community) and UVB induced changes on individual species within the community of S. epidermidis ( B ), S. hominis ( C ), C. acnes ( D ), M. luteus ( E ), and C. tuberculostearicum ( F ). Data are presented as mean ± SD. ** P < 0.01 and * P < 0.05, as determined via unpaired t -test.

Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

Techniques: Incubation

Journal: Applied and Environmental Microbiology

Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

doi: 10.1128/aem.01549-25

Figure Lengend Snippet: UVB exacerbates S. epidermidis -induced cytokine secretion, while S. hominis amplified secretion of IL-8 and CCL20 regardless of UVB exposure. NHEKs (p2–5) were incubated with media alone, S. hominis, or S. epidermidis for 30–60 min, media replaced with PBS, and exposed to UVB (0–33 mJ/cm²). Adherent bacteria were then co-cultured with NHEKs for 24 h, following which supernatants were collected to assess the effects on IL-6 ( A and E ), CCL20 ( B and F ), IL-8 ( C and G ), and TNF-α ( D and H ). Significance was assessed via one-way ANOVA followed by Tukey test, where ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

Techniques: Amplification, Incubation, Bacteria, Cell Culture

Journal: Applied and Environmental Microbiology

Article Title: Skin Staphylococcus species differentially modulate keratinocyte cytokine secretion in response to UVB

doi: 10.1128/aem.01549-25

Figure Lengend Snippet: S. hominis diminishes S. epidermidis- enhanced cytokine responses to UVR. NHEKs (p2–5) were incubated with media, S. hominis , S. epidermidis, or both for 30–60 min, media replaced with PBS, and subsequently exposed to UVB (0–20 mJ/cm²). Twenty-hours post-microbial inoculation, supernatants were collected and effects on cytokine secretion assessed by ELISA ( A–D ). **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05, as determined via one-way ANOVA followed by Tukey test.

Article Snippet: Pooled ( n = 3), normal human epidermal keratinocytes (NHEKs from juvenile foreskin, Promocell C-12005; passages 1–5) were routinely cultured in keratinocyte growth medium (KGM-2, Promocell).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal: Biomolecules

Article Title: Mandelamide Isolated from Prunus persica Flowers Attenuates TNF-α–Driven Oxidative and Inflammatory Responses in Human Skin Cells

doi: 10.3390/biom16050672

Figure Lengend Snippet: Effects of mandelamide ( 4 ) on pro-inflammatory mediator secretion in TNF-α–stimulated human epidermal keratinocytes (HEKs). Cells were pretreated with mandelamide at the indicated concentrations (12.5, 25, 50, and 100 μM) for 1 h, followed by stimulation with TNF-α for 12 or 24 h. The secretion levels of IL-6, IL-8, and IL-1β ( A ), as well as COX-2, PGE 2 , and nitric oxide (NO) ( B ), were quantified using ELISA, while NO production was additionally measured using the Griess assay. All experiments were performed according to the manufacturers’ protocols. Data are presented as mean ± SEM ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. control; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. TNF-α–treated group. Dexamethasone (Dexa) was used as a positive control in the IL-6 and IL-8 ELISAs.

Article Snippet: Human dermal fibroblasts (HDFs; CAT No. C-12302, adult donor) and human epidermal keratinocytes (HEKs; CAT No. C-12006, pooled adult donor) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Griess Assay, Control, Positive Control

Journal: Biomolecules

Article Title: Mandelamide Isolated from Prunus persica Flowers Attenuates TNF-α–Driven Oxidative and Inflammatory Responses in Human Skin Cells

doi: 10.3390/biom16050672

Figure Lengend Snippet: Effects of mandelamide ( 4 ) on the mRNA expression of MMPs and collagen-related genes in TNF-α/IFN-γ–stimulated human epidermal keratinocytes (HEKs). Cells were pretreated with mandelamide at the indicated concentrations (12.5, 25, 50, and 100 μM) for 1 h, followed by stimulation with TNF-α/IFN-γ for 24 h. The mRNA expression levels of MMP-1, MMP-2, MMP-9 ( A ), as well as COL1A1, COL1A2, COL3A1, and COL4A1 ( B ) were quantified by quantitative real-time PCR (qRT-PCR). All experiments were performed according to the manufacturers’ protocols. Data are presented as mean ± SEM ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test; ## p < 0.05 and ### p < 0.001 vs. control; *, **, and *** p < 0.05, p < 0.01, and p < 0.001 vs. TNF-α/IFN-γ–treated group.

Article Snippet: Human dermal fibroblasts (HDFs; CAT No. C-12302, adult donor) and human epidermal keratinocytes (HEKs; CAT No. C-12006, pooled adult donor) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Journal: Biomolecules

Article Title: Mandelamide Isolated from Prunus persica Flowers Attenuates TNF-α–Driven Oxidative and Inflammatory Responses in Human Skin Cells

doi: 10.3390/biom16050672

Figure Lengend Snippet: Effects of mandelamide ( 4 ) on hyaluronan synthase (HAS) gene expression in TNF-α/IFN-γ–stimulated human epidermal keratinocytes (HEKs). Cells were pretreated with mandelamide at the indicated concentrations (12.5, 25, 50, and 100 μM) for 1 h, followed by stimulation with TNF-α/IFN-γ for 24 h. The mRNA expression levels of HAS-1, HAS-2, and HAS-3 were quantified by quantitative real-time PCR (qRT-PCR). All experiments were performed according to the manufacturers’ protocols. Data are presented as mean ± SEM ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test; ## p < 0.05 and ### p < 0.001 vs. control; *, **, and *** p < 0.05, p < 0.01, and p < 0.001 vs. TNF-α/IFN-γ–treated group.

Article Snippet: Human dermal fibroblasts (HDFs; CAT No. C-12302, adult donor) and human epidermal keratinocytes (HEKs; CAT No. C-12006, pooled adult donor) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Gene Expression, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture restores gene expression of skin barrier molecules filaggrin and loricrin in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were left unstimulated (control) or stimulated with a psoriasis-like cytokine mixture (IL-1β, IL-17A, IL-22 and TNF-alpha, each 10 ng/mL) either in the absence (Psoriasis) or in the presence of the plant extract (Psoriasis + Extract) for 21 h. Gene expression levels of ( a ) FLG , ( b ) LOR and ( c ) IVL were determined by real-time PCR. Statistical significance was tested by a, c) one-way ANOVA with subsequent Sidak’s multiple comparison test or b) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12 * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Control, Real-time Polymerase Chain Reaction, Comparison

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture downregulates inflammatory markers in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of ( a ) IL1A , ( b ) IL1B, ( c ) IL1RN , ( d ) CXCL8 , ( e ) TNFA , ( f ) IL17C , ( g ) IL36G , ( h ) CSF2 , ( i ) VEGFA were measured. Statistical significance was tested by a, b, c, e, h) one-way ANOVA with subsequent Sidak’s multiple comparison test or d, f, g) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12, * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant). Protein expression levels of ( j ) IL-8 and k) TNFα were measured in the supernatants of the cells by ELISA. Values for unstimulated control and plant extract-treated cells were below the detection limit of 31,3 pg/mL (IL-8) or 62,5 pg/mL (TNFα) and not detectable; therefore, no statistical analysis was performed. n.d.= non-detectable.

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Comparison, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture lowers upregulated antimicrobial peptide expression in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of ( a ) S100A7 , ( c ) DEFB4A and ( e ) DEFB103A were measured. Statistical significance was tested by ( a , c ) one-way ANOVA with subsequent Sidak’s multiple comparison test or ( e ) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12). Protein expression levels of ( b ) psoriasin and ( d ) hBD2 were measured in the supernatants of the cells by ELISA. Statistical significance was tested by b) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12) or d) one-way ANOVA with subsequent Sidak’s multiple comparison test ( n = 6, * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Expressing, Gene Expression, Comparison, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture reduces NFKBIZ and NFKBIA gene expression and IκBζ protein levels. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of ( a ) NFKBIZ and ( c ) NFKBIA were measured and statistical significance was tested by one-way ANOVA with subsequent Sidak’s multiple comparisons test ( n = 12; * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant). ( b ) Protein expression levels of IκBζ were assessed by western blot using an IκBζ antibody. Detection of pan-actin serves as a loading control. Uncropped blots are shown in Supplementary Figure .

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture activates the AhR in the 2D psoriasis model. ( a ) CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of CYP1A1 were measured and statistical significance was tested by Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12). ( b ) To determine AhR activation, NHEKs were transfected with the pGudLuc6.1 plasmid containing firefly luciferase which expression depends on AhR activation and the pGL4.74 [ hRLuc /TK] reference plasmid containing renilla luciferase. One day after transfection, cells were stimulated as described in ( a ). After cell lysis, activation of AhR was determined by measurement of relative luciferase activities. Statistical significance was tested by one-way ANOVA with subsequent Sidak’s multiple comparison test ( n = 12; ** p < 0.01; *** p < 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Activation Assay, Transfection, Plasmid Preparation, Luciferase, Expressing, Lysis, Comparison

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Downregulation of AhR inhibits the filaggrin-inducing effects but not the anti-inflammatory effects of the plant extract in the 2D psoriasis model. NHEKs were transfected with control or AhR siRNA and stimulated as described in Fig. . Gene expression levels of ( a ) CYP1A1 , ( b ) FLG , ( c ) NFKBIZ , ( d ) TNFA , ( e ) IL36G ( f ) CXCL8 and ( g ) DEFB4A were measured. Statistical significance was determined by ( a – f ) one-way ANOVA with subsequent Sidak’s multiple comparison test or ( c ) Kruskal-Wallis with subsequent Dunn’s multiple comparison test (n = 9; * p < 0.05; ** p < 0.01; *** p 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Transfection, Control, Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: The plant extract mixture exhibits antioxidant effects in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . ( a ) Gene expression levels of NQO1 were measured and statistical significance was tested by one-way ANOVA with subsequent Sidak’s multiple comparisons test ( n = 12). ( b ) Intracellular reactive oxygen species were measured by DCFDA-based assay. The mean fluorescence value of the control cells was set to 100%, and the relative intracellular ROS levels of the other samples were calculated as percentages of the control. Statistical significance was tested by Kruskal-Wallis test with subsequent Dunn’s multiple comparison test ( n = 12, * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant). ( c ) NHEKs were transfected and stimulated as described in Fig. . Gene expression levels of NQO1 were measured and statistical significance was determined by one-way ANOVA with subsequent Sidak’s multiple comparison test ( n = 9; * p < 0.05; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Fluorescence, Control, Comparison, Transfection

Journal: International Journal of Molecular Sciences

Article Title: Activity and Biocompatibility Evaluation of Enzybiotic Compositions Formulated with Azotobacter vinelandii Alginate for Topical Use

doi: 10.3390/ijms27093856

Figure Lengend Snippet: HaCaT cell viability in the presence of gel components and the composition. bALG, bacterial sodium alginate; bALG-Lys, composition of sodium alginate (2% initial concentration) and LysSi3-LK (0.5 mg/mL initial concentration). The mean values for three replicates are shown for all groups of samples (± SD). *— p < 0.05, ****— p < 0.0001 compared to bALG-Lys group, two-way ANOVA.

Article Snippet: HaCaT keratinocytes (ATCC PCS-200-011) were seeded into a 96-well plate for adherent cultures at a density of 20,000 cells/well.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Activity and Biocompatibility Evaluation of Enzybiotic Compositions Formulated with Azotobacter vinelandii Alginate for Topical Use

doi: 10.3390/ijms27093856

Figure Lengend Snippet: Results of the migration assay of HaCaT cells with the investigated preparations within 48 h. Scratch closure area, which was occupied by the cells for: ( a ) Bacterial sodium alginate gel samples; ( b ) Bacterial calcium alginate hydrogel samples. Data are presented as mean values ± SD. ( c ) Bright field light microscopy (scale bar, 200 μm). Na-ALG, bacterial sodium alginate gels; Ca-ALG, bacterial calcium alginate hydrogels; Lys, endolysin LysSi3-LK. **— p < 0.01, ***— p < 0.001 compared to bALG group, one-way ANOVA.

Article Snippet: HaCaT keratinocytes (ATCC PCS-200-011) were seeded into a 96-well plate for adherent cultures at a density of 20,000 cells/well.

Techniques: Migration, Light Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Activity and Biocompatibility Evaluation of Enzybiotic Compositions Formulated with Azotobacter vinelandii Alginate for Topical Use

doi: 10.3390/ijms27093856

Figure Lengend Snippet: HaCaT cells viability dynamics in the biocompatibility assay: ( a ) Viable cells percent, mean values are presented ± SD; ( b ) Fluorescence microscopy of HaCaT incorporated into bALG calcium-alginate films with addition of the endolysin LysSi3-LK in 0.25 and 0.5 μg/mL concentrations. *— p < 0.05; **— p < 0.01, two-way ANOVA.

Article Snippet: HaCaT keratinocytes (ATCC PCS-200-011) were seeded into a 96-well plate for adherent cultures at a density of 20,000 cells/well.

Techniques: Fluorescence, Microscopy